Environmental Genomics™

Anaerobic Digester Sample

May 14, 2024
52.3% Bacteria/40.1% Archaea^*
^* estimated by qPCR

AOB	NOB	NRB	SOB	SRB	PAO	GAO	EPS	FOA	FIL	C1s	CH₄
0.0%	0.0%	0.58%	0.03%	0.01%	0.19%	0.63%	0.02%	0.01%	0.64%	0.44%	39.3%

Archaea

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Genus	%	CH₄	FIL
Methanomassiliicoccus	17.2	Methylotrophic
Methanothermobacter	16.3	Hydrogenotrophic
Methanothrix	4.0	Acetoclastic	●
Methanosarcina	1.2	Acetoclastic-Methylotrophic
midas_g_90791	0.4
Methanospirillum	0.4	Hydrogenotrophic
Methanosphaerula	0.2
midas_g_118835	0.2
Methanoculleus	0.1	Hydrogenotrophic
Methanoregula	0.1	Hydrogenotrophic
midas_g_90473	0.1
Methanolinea	0.0	Hydrogenotrophic	●
Methanocorpusculum	0.0	Hydrogenotrophic
Methanomethylovorans	0.0	Methylotrophic
midas_g_91627	0.0
Methanofollis	0.0	Hydrogenotrophic
midas_g_9568	0.0
Ca_Methanogranum	0.0
Ca_Nitrocosmicus	0.0
midas_g_92967	0.0
Ca_Methanofastidiosum	0.0	Unique Pathway
Methanolobus	0.0	Methylotrophic
midas_g_91233	0.0
midas_g_110826	0.0
Methanobacterium	0.0	Hydrogenotrophic
RumEn_M2	0.0
Ca_Methanoplasma	0.0
midas_g_90441	0.0
Methanosphaera	0.0	Methylotrophic

Bacteria

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Genus	%	NRB	PAO	GAO	FIL	C1S
Lentimicrobium	5.2
Rhodobacter	0.8
Defluviicoccus	0.4			●	●
Methylotenera	0.4					●
Galbitalea	0.3
Romboutsia	0.3
Limnohabitans	0.2
Comamonas	0.2
Diaphorobacter	0.2	●
Microbacterium	0.2
Micropruina	0.2			●
Clostridium_sensu_stricto_1	0.2
midas_g_33	0.1
AAP99	0.1
IMCC26207	0.1
Lautropia	0.1	●
Rhizobacter	0.1
midas_g_81	0.1
Devosia	0.1		●
Propioniciclava	0.1
midas_g_1929	0.1
Rhodoferax	0.1
Pseudoxanthomonas	0.1
Lysinibacillus	0.1
Streptococcus	0.1				●
Pseudomonas	0.1	●
midas_g_153	0.1
Amaricoccus	0.1
Megasphaera	0.1
Ferruginibacter	0.1
Dokdonella	0.1	●	●

Taxonomy Chart

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All Assignments

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Kingdom	Phylum	Class	Order	Family	Genus	%
Bacteria	Actinobacteriota	Acidimicrobiia	Microtrichales	Microtrichaceae	IMCC26207	0.14
Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Intrasporangiaceae	Ca_Phosphoribacter	0.8
Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Galbitalea	0.32
Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Microbacterium	0.2
Bacteria	Actinobacteriota	Actinobacteria	Propionibacteriales	Propionibacteriaceae	Micropruina	0.19
Bacteria	Actinobacteriota	Actinobacteria	Propionibacteriales	Propionibacteriaceae	Propioniciclava	0.11
Bacteria	Actinobacteriota	Coriobacteriia	Coriobacteriales	Atopobiaceae	Atopobium	0.16
Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	Lentimicrobiaceae	Lentimicrobium	5.15
Bacteria	Caldatribacteriota	Caldatribacteriia	Caldatribacteriales	Caldatribacteriaceae	Ca_Caldatribacterium	0.31
Bacteria	Campylobacterota	Campylobacteria	Campylobacterales	Helicobacteraceae	Wolinella	0.65
Bacteria	Chloroflexi	Anaerolineae	RBG-13-54-9	Ca_Brachytrichaceae	midas_g_1929	0.1
Bacteria	Chloroflexi	Anaerolineae	SBR1031	midas_f_184	midas_g_184	0.36
Bacteria	Coprothermobacterota	Coprothermobacteria	Coprothermobacterales	Coprothermobacteraceae	Coprothermobacter	4.57
Bacteria	Firmicutes	Bacilli	Erysipelotrichales	Erysipelotrichaceae	Solobacterium	0.19
Bacteria	Firmicutes	Bacilli	Haloplasmatales	Haloplasmataceae	Haloplasma	0.14
Bacteria	Firmicutes	Bacilli	Izemoplasmatales	midas_f_16343	midas_g_84542	0.26
Bacteria	Firmicutes	Clostridia	Caldicoprobacterales	Caldicoprobacteraceae	Caldicoprobacter	2.09
Bacteria	Firmicutes	Clostridia	Caldicoprobacterales	Caldicoprobacteraceae	midas_g_9339	0.35
Bacteria	Firmicutes	Clostridia	Clostridiales	Clostridiaceae	Clostridium_sensu_stricto_1	0.18
Bacteria	Firmicutes	Clostridia	Eubacteriales	Eubacteriaceae	midas_g_2229	0.11
Bacteria	Firmicutes	Clostridia	Gracilibacteraceae	Lutispora	midas_g_2455	0.14
Bacteria	Firmicutes	Clostridia	Lachnospirales	Defluviitaleaceae	Defluviitalea	0.88
Bacteria	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Anaerocolumna	0.2
Bacteria	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Herbinix	0.28
Bacteria	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	midas_g_116023	0.11
Bacteria	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	midas_g_99962	0.1
Bacteria	Firmicutes	Clostridia	Oscillospirales	Eubacterium_coprostanoligenes_group	midas_g_99032	0.28
Bacteria	Firmicutes	Clostridia	Oscillospirales	Hungateiclostridiaceae	Clostridium_sp._JC3	0.75
Bacteria	Firmicutes	Clostridia	Oscillospirales	Hungateiclostridiaceae	Ruminiclostridium	0.51
Bacteria	Firmicutes	Clostridia	Oscillospirales	Hungateiclostridiaceae	Thermoclostridium	0.63
Bacteria	Firmicutes	Clostridia	Oscillospirales	Hungateiclostridiaceae	midas_g_2604	0.21
Bacteria	Firmicutes	Clostridia	Oscillospirales	Hungateiclostridiaceae	midas_g_7266	0.2
Bacteria	Firmicutes	Clostridia	Oscillospirales	UCG-010	midas_g_583	0.2
Bacteria	Firmicutes	Clostridia	Peptostreptococcales-Tissierellales	Family_XI	Tepidimicrobium	0.41
Bacteria	Firmicutes	Clostridia	Peptostreptococcales-Tissierellales	Peptostreptococcaceae	Romboutsia	0.27
Bacteria	Firmicutes	Clostridia	Peptostreptococcales-Tissierellales	Thermotaleaceae	midas_g_91945	0.35
Bacteria	Firmicutes	Clostridia	Proteinivoracales	midas_f_1799	midas_g_1799	0.2
Bacteria	Firmicutes	D8A-2	midas_o_13	midas_f_13	midas_g_1361	0.71
Bacteria	Firmicutes	D8A-2	midas_o_13	midas_f_13	midas_g_554	2.56
Bacteria	Firmicutes	Desulfotomaculia	Desulfotomaculales	Pelotomaculaceae	Pelotomaculum	0.89
Bacteria	Firmicutes	Dethiobacteria	Dethiobacterales	Dethiobacteraceae	midas_g_1900	0.32
Bacteria	Firmicutes	Dethiobacteria	Dethiobacterales	Dethiobacteraceae	midas_g_8028	0.2
Bacteria	Firmicutes	Dethiobacteria	Dethiobacterales	Dethiobacteraceae	midas_g_88	1.56
Bacteria	Firmicutes	Limnochordia	B55-F-B-G02	midas_f_3498	midas_g_94400	0.28
Bacteria	Firmicutes	Limnochordia	Hydrogenispora	midas_f_2701	midas_g_3323	0.15
Bacteria	Firmicutes	Limnochordia	Hydrogenispora	midas_f_2701	midas_g_3406	0.27
Bacteria	Firmicutes	Limnochordia	Hydrogenispora	midas_f_2701	midas_g_92005	0.17
Bacteria	Firmicutes	Limnochordia	Limnochordales	Limnochordaceae	midas_g_93815	0.16
Bacteria	Firmicutes	Limnochordia	M55-D21	Moorella_glycerini	midas_g_2860	0.5
Bacteria	Firmicutes	Limnochordia	MBA03	midas_f_112	midas_g_112	6.67
Bacteria	Firmicutes	Limnochordia	MBA03	midas_f_112	midas_g_2506	0.43
Bacteria	Firmicutes	Limnochordia	midas_o_1979	midas_f_1979	midas_g_1979	0.38
Bacteria	Firmicutes	Syntrophomonadia	Syntrophomonadales	Syntrophomonadaceae	Syntrophomonas	0.26
Bacteria	Firmicutes	Thermacetogenia	Thermacetogeniales	Thermacetogeniaceae	Syntrophaceticus	5.91
Bacteria	Firmicutes	Thermovenabulia	Thermovenabulales	Tepidanaerobacter	midas_g_1240	0.12
Bacteria	Firmicutes	midas_c_4	DTU014	midas_f_148	midas_g_148	0.64
Bacteria	Firmicutes	midas_c_4	DTU014	midas_f_4221	midas_g_4221	1.29
Bacteria	Firmicutes	midas_c_4	DTU014	midas_f_80	midas_g_80	1.38
Bacteria	Hydrothermae	midas_c_2121	midas_o_2121	midas_f_2121	midas_g_2121	0.22
Bacteria	Planctomycetota	Phycisphaerae	Pla1_lineage	midas_f_3438	midas_g_4387	0.11
Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	Thermogutta	0.13
Bacteria	Proteobacteria	Alphaproteobacteria	Defluviicoccales	Defluviicoccaceae	Defluviicoccus	0.45
Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Devosiaceae	Devosia	0.12
Bacteria	Proteobacteria	Alphaproteobacteria	Rhodobacterales	Rhodobacteraceae	Rhodobacter	0.83
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Burkholderiaceae	Lautropia	0.14
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Acidovorax	0.2
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Comamonas	0.2
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Diaphorobacter	0.2
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Limnohabitans	0.22
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Rhizobacter	0.13
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Rhodoferax	0.1
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	midas_g_33	0.15
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	midas_g_81	0.12
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Methylophilaceae	Methylotenera	0.41
Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Sutterellaceae	AAP99	0.15
Bacteria	Synergistota	Synergistia	Synergistales	Synergistaceae	Acetomicrobium	0.91
Bacteria	Thermotogota	Thermotogae	Thermotogales	Fervidobacteriaceae	Fervidobacterium	0.59
Archaea	Crenarchaeota	Bathyarchaeia	midas_o_90791	midas_f_118835	midas_g_118835	0.16
Archaea	Crenarchaeota	Bathyarchaeia	midas_o_90791	midas_f_90791	midas_g_90791	0.44
Archaea	Euryarchaeota	Methanobacteria	Methanobacteriales	Methanothermobacteriaceae	Methanothermobacter	16.35
Archaea	Halobacterota	Methanomicrobia	Methanomicrobiales	Methanomicrobiaceae	Methanoculleus	0.13
Archaea	Halobacterota	Methanomicrobia	Methanomicrobiales	Methanoregulaceae	Methanosphaerula	0.23
Archaea	Halobacterota	Methanomicrobia	Methanomicrobiales	Methanospirillaceae	Methanospirillum	0.4
Archaea	Halobacterota	Methanosarcinia	Methanosarciniales	Methanosaetaceae	Methanothrix	4.01
Archaea	Halobacterota	Methanosarcinia	Methanosarciniales	Methanosarcinaceae	Methanosarcina	1.18
Archaea	Thermoplasmatota	Thermoplasmata	Methanomassiliicoccales	Methanomassiliicoccaceae	Methanomassiliicoccus	17.23

Higher Taxonomic Levels

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Kingdom	% of reads
Archaea	40.13
Bacteria	52.33

Phylum	% of reads
Actinobacteriota	1.92
Bacteroidota	5.15
Caldatribacteriota	0.31
Campylobacterota	0.65
Chloroflexi	0.46
Coprothermobacterota	4.57
Crenarchaeota	0.6
Euryarchaeota	16.35
Firmicutes	33.89
Halobacterota	5.95
Hydrothermae	0.22
Planctomycetota	0.24
Proteobacteria	3.42
Synergistota	0.91
Thermoplasmatota	17.23
Thermotogota	0.59

Class	% of reads
Acidimicrobiia	0.14
Actinobacteria	1.62
Alphaproteobacteria	1.4
Anaerolineae	0.46
Bacilli	0.59
Bacteroidia	5.15
Bathyarchaeia	0.6
Caldatribacteriia	0.31
Campylobacteria	0.65
Clostridia	8.45
Coprothermobacteria	4.57
Coriobacteriia	0.16
D8A-2	3.27
Desulfotomaculia	0.89
Dethiobacteria	2.08
Gammaproteobacteria	2.02
Limnochordia	9.01
Methanobacteria	16.35
Methanomicrobia	0.76
Methanosarcinia	5.19
Phycisphaerae	0.11
Planctomycetes	0.13
Synergistia	0.91
Syntrophomonadia	0.26
Thermacetogenia	5.91
Thermoplasmata	17.23
Thermotogae	0.59
Thermovenabulia	0.12
midas_c_2121	0.22
midas_c_4	3.31

Order	% of reads
B55-F-B-G02	0.28
Burkholderiales	2.02
Caldatribacteriales	0.31
Caldicoprobacterales	2.44
Campylobacterales	0.65
Clostridiales	0.18
Coprothermobacterales	4.57
Coriobacteriales	0.16
DTU014	3.31
Defluviicoccales	0.45
Desulfotomaculales	0.89
Dethiobacterales	2.08
Erysipelotrichales	0.19
Eubacteriales	0.11
Gracilibacteraceae	0.14
Haloplasmatales	0.14
Hydrogenispora	0.59
Izemoplasmatales	0.26
Lachnospirales	1.57
Limnochordales	0.16
M55-D21	0.5
MBA03	7.1
Methanobacteriales	16.35
Methanomassiliicoccales	17.23
Methanomicrobiales	0.76
Methanosarciniales	5.19
Micrococcales	1.32
Microtrichales	0.14
Oscillospirales	2.78
Peptostreptococcales-Tissierellales	1.03
Pirellulales	0.13
Pla1_lineage	0.11
Propionibacteriales	0.3
Proteinivoracales	0.2
RBG-13-54-9	0.1
Rhizobiales	0.12
Rhodobacterales	0.83
SBR1031	0.36
Sphingobacteriales	5.15
Synergistales	0.91
Syntrophomonadales	0.26
Thermacetogeniales	5.91
Thermotogales	0.59
Thermovenabulales	0.12
midas_o_13	3.27
midas_o_1979	0.38
midas_o_2121	0.22
midas_o_90791	0.6

Family	% of reads
Atopobiaceae	0.16
Burkholderiaceae	0.14
Ca_Brachytrichaceae	0.1
Caldatribacteriaceae	0.31
Caldicoprobacteraceae	2.44
Clostridiaceae	0.18
Comamonadaceae	1.32
Coprothermobacteraceae	4.57
Defluviicoccaceae	0.45
Defluviitaleaceae	0.88
Dethiobacteraceae	2.08
Devosiaceae	0.12
Erysipelotrichaceae	0.19
Eubacteriaceae	0.11
Eubacterium_coprostanoligenes_group	0.28
Family_XI	0.41
Fervidobacteriaceae	0.59
Haloplasmataceae	0.14
Helicobacteraceae	0.65
Hungateiclostridiaceae	2.3
Intrasporangiaceae	0.8
Lachnospiraceae	0.69
Lentimicrobiaceae	5.15
Limnochordaceae	0.16
Lutispora	0.14
Methanomassiliicoccaceae	17.23
Methanomicrobiaceae	0.13
Methanoregulaceae	0.23
Methanosaetaceae	4.01
Methanosarcinaceae	1.18
Methanospirillaceae	0.4
Methanothermobacteriaceae	16.35
Methylophilaceae	0.41
Microbacteriaceae	0.52
Microtrichaceae	0.14
Moorella_glycerini	0.5
Pelotomaculaceae	0.89
Peptostreptococcaceae	0.27
Pirellulaceae	0.13
Propionibacteriaceae	0.3
Rhodobacteraceae	0.83
Sutterellaceae	0.15
Synergistaceae	0.91
Syntrophomonadaceae	0.26
Tepidanaerobacter	0.12
Thermacetogeniaceae	5.91
Thermotaleaceae	0.35
UCG-010	0.2
midas_f_112	7.1
midas_f_118835	0.16
midas_f_13	3.27
midas_f_148	0.64
midas_f_16343	0.26
midas_f_1799	0.2
midas_f_184	0.36
midas_f_1979	0.38
midas_f_2121	0.22
midas_f_2701	0.59
midas_f_3438	0.11
midas_f_3498	0.28
midas_f_4221	1.29
midas_f_80	1.38
midas_f_90791	0.44

Genus	% of reads
AAP99	0.15
Acetomicrobium	0.91
Acidovorax	0.2
Anaerocolumna	0.2
Atopobium	0.16
Ca_Caldatribacterium	0.31
Ca_Phosphoribacter	0.8
Caldicoprobacter	2.09
Clostridium_sensu_stricto_1	0.18
Clostridium_sp._JC3	0.75
Comamonas	0.2
Coprothermobacter	4.57
Defluviicoccus	0.45
Defluviitalea	0.88
Devosia	0.12
Diaphorobacter	0.2
Fervidobacterium	0.59
Galbitalea	0.32
Haloplasma	0.14
Herbinix	0.28
IMCC26207	0.14
Lautropia	0.14
Lentimicrobium	5.15
Limnohabitans	0.22
Methanoculleus	0.13
Methanomassiliicoccus	17.23
Methanosarcina	1.18
Methanosphaerula	0.23
Methanospirillum	0.4
Methanothermobacter	16.35
Methanothrix	4.01
Methylotenera	0.41
Microbacterium	0.2
Micropruina	0.19
Pelotomaculum	0.89
Propioniciclava	0.11
Rhizobacter	0.13
Rhodobacter	0.83
Rhodoferax	0.1
Romboutsia	0.27
Ruminiclostridium	0.51
Solobacterium	0.19
Syntrophaceticus	5.91
Syntrophomonas	0.26
Tepidimicrobium	0.41
Thermoclostridium	0.63
Thermogutta	0.13
Wolinella	0.65
midas_g_112	6.67
midas_g_116023	0.11
midas_g_118835	0.16
midas_g_1240	0.12
midas_g_1361	0.71
midas_g_148	0.64
midas_g_1799	0.2
midas_g_184	0.36
midas_g_1900	0.32
midas_g_1929	0.1
midas_g_1979	0.38
midas_g_2121	0.22
midas_g_2229	0.11
midas_g_2455	0.14
midas_g_2506	0.43
midas_g_2604	0.21
midas_g_2860	0.5
midas_g_33	0.15
midas_g_3323	0.15
midas_g_3406	0.27
midas_g_4221	1.29
midas_g_4387	0.11
midas_g_554	2.56
midas_g_583	0.2
midas_g_7266	0.2
midas_g_80	1.38
midas_g_8028	0.2
midas_g_81	0.12
midas_g_84542	0.26
midas_g_88	1.56
midas_g_90791	0.44
midas_g_91945	0.35
midas_g_92005	0.17
midas_g_9339	0.35
midas_g_93815	0.16
midas_g_94400	0.28
midas_g_99032	0.28
midas_g_99962	0.1

Aster Bio, Inc.

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Houston, TX 77227-7841

+1 713 724-0082

Send samples to:

Aster Bio, Inc.
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